Insertion junction: LMJ.RY0402.106376_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.106376
Chromosome:chromosome 17
Location:7091747
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g747247 FAP2 TPR-domain Flagellar Associated Protein 2; (1 of 1) IPR002151//IPR003593//IPR013026//IPR019734//IPR027417 - Kinesin light chain // AAA+ ATPase domain // Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAATGGAACCCCCTCTTCATCCGCCACCT
Internal bar code:TGTTGAGTCCCCTTGGAGATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:55
LEAP-Seq percent confirming:92.402
LEAP-Seq n confirming:377
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTAGGGTGGATTGGAGGTGA
Suggested primer 2:ACATTGCGAGCAAGACAGTG