Insertion junction: LMJ.RY0402.106376_4

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.106376
Chromosome:chromosome 17
Location:7091754
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g747247 FAP2 TPR-domain Flagellar Associated Protein 2; (1 of 1) IPR002151//IPR003593//IPR013026//IPR019734//IPR027417 - Kinesin light chain // AAA+ ATPase domain // Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAGGGGGATGCGGAAGGGGCACACGCGG
Internal bar code:AGAGGGTGTGATCGTCTAGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:90
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:308
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TACCAGCCCAAACTAAACCG
Suggested primer 2:ACATTGCGAGCAAGACAGTG