| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.106428 |
| Chromosome: | chromosome_6 |
| Location: | 7207088 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g297850 | DHC12 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGCGCTCAGTGGGTCCACGTCAGCAGGCA |
| Internal bar code: | AGACCGTAGCAGTCAGTTAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 601 |
| LEAP-Seq percent confirming: | 64.8336 |
| LEAP-Seq n confirming: | 448 |
| LEAP-Seq n nonconfirming: | 243 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAATGGCGTCAAAGTCCAC |
| Suggested primer 2: | ATACCATGCCCGAAGATGAG |