| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.106513 |
| Chromosome: | chromosome 11 |
| Location: | 3022826 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478600 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCCGGGCACGTTGACATTAACGCCCAC |
| Internal bar code: | AACGACTGCCGTGTAGGGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 99.4503 |
| LEAP-Seq n confirming: | 2714 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCCAGCCTTCCTAGATT |
| Suggested primer 2: | ATCGGGAGGTTACATTGCTG |