Insertion junction: LMJ.RY0402.106539_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.106539
Chromosome:chromosome 16
Location:5182642
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g683100 (1 of 239) IPR016024 - Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCACAGTTTCAAAGCCATGCCTGCGGCAT
Internal bar code:CCACGCCCAGAAGCACGGGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:516
LEAP-Seq percent confirming:99.3883
LEAP-Seq n confirming:6987
LEAP-Seq n nonconfirming:43
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTTGATACCTGGGTGCAT
Suggested primer 2:GTAGCTCAAGGCCCAGAGTG