| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.106591 |
| Chromosome: | chromosome 17 |
| Location: | 2575884 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716750 | (1 of 77) IPR029071 - Ubiquitin-related domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGTACCGTATCTTAACAACACGCCAGT |
| Internal bar code: | TGGACAGAGGAGGGTATGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 673 |
| LEAP-Seq percent confirming: | 99.4489 |
| LEAP-Seq n confirming: | 2346 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGATGTGAACTACCCGCT |
| Suggested primer 2: | ATACGCCTCCGTACACGTTC |