| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.106596 |
| Chromosome: | chromosome 12 |
| Location: | 9258028 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541400 | (1 of 5) PF04366 - Las17-binding protein actin regulator (Ysc84) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCATGAAGGAGTCGCTGACGGTGATGAC |
| Internal bar code: | GAGTGCACGACACTTACAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 98.9988 |
| LEAP-Seq n confirming: | 1681 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGGCAATCGTGTGGAGT |
| Suggested primer 2: | TCACTCACAGCCTCCAACTG |