| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106615 |
| Chromosome: | chromosome 13 |
| Location: | 3379850 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAAATCAAGTGTCACTTATCTATGAGTT |
| Internal bar code: | TTCTCGAGAGATGATCCGCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 430 |
| LEAP-Seq percent confirming: | 99.2941 |
| LEAP-Seq n confirming: | 1688 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCATACGAGAGGGAGAG |
| Suggested primer 2: | ATCCGACACGGACTTACTGG |