Insertion junction: LMJ.RY0402.106643_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.106643
Chromosome:chromosome 1
Location:6070257
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g043250 (1 of 3) PTHR30570:SF0 - PHOSPHATE-BINDING PROTEIN PSTS CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGTCCACCGCTTTCGCATTTGGCTACGC
Internal bar code:ATGGATGTCCTCCGACGCACGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:546
LEAP-Seq percent confirming:98.5255
LEAP-Seq n confirming:3675
LEAP-Seq n nonconfirming:55
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CCACAAACTGTCCTCCCACT
Suggested primer 2:GTGGCCTGTGACTTACCGTT