Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.106679 |
Chromosome: | chromosome_3 |
Location: | 3577828 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g168200 | FAP69 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGTTTTTGGGGATGGAGGAGCGGGTTGGAG |
Internal bar code: | CAAGGGCCGTAAAGACGTCCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 581 |
LEAP-Seq percent confirming: | 98.0532 |
LEAP-Seq n confirming: | 3425 |
LEAP-Seq n nonconfirming: | 68 |
LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTACCTCTCGCTTAGCCCCT |
Suggested primer 2: | CATTTCATTCGTGCATACGG |