| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106691 |
| Chromosome: | chromosome 10 |
| Location: | 2622667 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437300 | (1 of 1) PF00053 - Laminin EGF domain (Laminin_EGF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGAGGCATGCACGACCCTATCACGCTGC |
| Internal bar code: | ATGCAAGCTAAGCTCGCTCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTTGTGCGGAAATGGACT |
| Suggested primer 2: | CACTGTGTTCTCTCGCCAAA |