| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.106757 |
| Chromosome: | chromosome 2 |
| Location: | 4703971 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101850 | NAT11 | (1 of 1) PTHR23091:SF46 - N-ALPHA-ACETYLTRANSFERASE 50; N-acetyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGTGCCCATGAGCTCGCCACACAAGCT |
| Internal bar code: | AAGCTACCGCAATTAGGATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 399 |
| LEAP-Seq percent confirming: | 98.6797 |
| LEAP-Seq n confirming: | 2616 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCTATCGGTGAGTGTGAG |
| Suggested primer 2: | CCAAAAATGCTTCCGTGAGT |