| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106779 |
| Chromosome: | chromosome 16 |
| Location: | 4589935 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687900 | LHCA7 | Light-harvesting protein of photosystem I; (1 of 3) K08911 - light-harvesting complex I chlorophyll a/b binding protein 5 (LHCA5) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCATTGGTGCCAAGGTTGGCCTGGGCT |
| Internal bar code: | GGTTTTCTAAAACCGAGCCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 376 |
| LEAP-Seq percent confirming: | 99.3421 |
| LEAP-Seq n confirming: | 151 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGAACTCCTCGGTGAAGC |
| Suggested primer 2: | TGGCACCTTGTATGGACAAA |