| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.106795 |
| Chromosome: | chromosome 12 |
| Location: | 5606831 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532000 | (1 of 3) PF04359 - Protein of unknown function (DUF493) (DUF493) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGAAAGCCGGCGTGGAAGGCTGGAAGC |
| Internal bar code: | GTCGGCACCGCGTATCGCACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 548 |
| LEAP-Seq percent confirming: | 99.5088 |
| LEAP-Seq n confirming: | 2026 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTAGGTTGCACCTGCTGA |
| Suggested primer 2: | GGTGTCCACGCTTTACCTGT |