| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106839 |
| Chromosome: | chromosome 13 |
| Location: | 4406535 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603100 | GT90-48,GT90F48 | GT90 family protein 48; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGCACCGTGTCCGTGGGAGGTGCTTCG |
| Internal bar code: | CTGTCGCGACTTCGGTTCTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 99.3007 |
| LEAP-Seq n confirming: | 568 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGAGAACTCCACATCGGG |
| Suggested primer 2: | TACACACATGCGAGCACTGA |