| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106845 |
| Chromosome: | chromosome 6 |
| Location: | 1026635 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g256200 | HAT3 | Histone acetyltransferase 3; (1 of 1) K06062 - histone acetyltransferase (PCAF, KAT2, GCN5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTCAGGCAGGCTCGCATATCTGCTGGT |
| Internal bar code: | ATGGCCTCGCCTGGATAAATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 960 |
| LEAP-Seq percent confirming: | 99.7281 |
| LEAP-Seq n confirming: | 1467 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGGTGGTTGAGGTCAAGT |
| Suggested primer 2: | CTCCCTTCCACCTTCTTTCC |