| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106850 |
| Chromosome: | chromosome 6 |
| Location: | 3182126 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275650 | RPN3 | (1 of 1) K03033 - 26S proteasome regulatory subunit N3 (PSMD3, RPN3); 26S proteasome regulatory subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTCTTGGTTCAAGGAATGGAAGAAGCTC |
| Internal bar code: | ACGCGATGTCTGCGTCAGCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 99.6438 |
| LEAP-Seq n confirming: | 3077 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTTCCTGCCTTTGTTGC |
| Suggested primer 2: | GGACGAGGACGACTTCTGAG |