| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.106871 |
| Chromosome: | chromosome_6 |
| Location: | 2800741 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g271200 | PNO1 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGCCTGGTAACAAGCTTTTACTCGAGCGC |
| Internal bar code: | GGGGCTTCCGTGATCTTCACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 99.1758 |
| LEAP-Seq n confirming: | 1805 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCAGGGGAATATTAAGGA |
| Suggested primer 2: | TTTGGATTGTGCTCAAGCTG |