| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.106892 |
| Chromosome: | chromosome_11 |
| Location: | 2082086 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g469000 | FAP107 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGAGAGCGAGGCGCTCGGGTGGGTGCGGA |
| Internal bar code: | GAACTTGGCCGGGCATCGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 753 |
| LEAP-Seq percent confirming: | 98.9395 |
| LEAP-Seq n confirming: | 2519 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACTTAGAAGCATAGGCGG |
| Suggested primer 2: | TTGTGCTAGACAGCACGACC |