| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.106892 |
| Chromosome: | chromosome 11 |
| Location: | 2082090 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g469000 | FAP107 | Flagellar Associated Protein 107 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGAGCTATCCTCACACCCGCACGACCGC |
| Internal bar code: | TTGGACTGGTATGTAATTACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 914 |
| LEAP-Seq percent confirming: | 99.787 |
| LEAP-Seq n confirming: | 5153 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACTTAGAAGCATAGGCGG |
| Suggested primer 2: | TTGTGCTAGACAGCACGACC |