| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.106922 |
| Chromosome: | chromosome 14 |
| Location: | 1216378 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616151 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCGCACACACTCAAACTCACACTCCTCG |
| Internal bar code: | GTGCGTACGCCATCAAGCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 578 |
| LEAP-Seq percent confirming: | 95.7965 |
| LEAP-Seq n confirming: | 1732 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGATCGCTTTCGGTACCTC |
| Suggested primer 2: | GGGTGGGGTCTGGAACTATT |