Insertion junction: LMJ.RY0402.106925_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.106925
Chromosome:chromosome 4
Location:1730902
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g212401 UBC15 (1 of 2) K10586 - baculoviral IAP repeat-containing protein 6 (apollon) (BIRC6, BRUCE); E2 Ubiquitin conjugating enzyme intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTCTGCTGCGCTTGCATGTGTCATAAGA
Internal bar code:TCGAACCGAATCGGGCGGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:657
LEAP-Seq percent confirming:99.1228
LEAP-Seq n confirming:113
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGGATGCACTGACGACTG
Suggested primer 2:GCGTGTGTGTGTGTGTGTGT