| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.106936 |
| Chromosome: | chromosome 13 |
| Location: | 588983 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565550 | RPB11 | DNA-directed RNA polymerase II, 13.6 kDa polypeptide | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGGAGTGAAGGTTGCGGCAACCAGTTG |
| Internal bar code: | CGCGATGAAGTACGGAACGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 96.9443 |
| LEAP-Seq n confirming: | 2665 |
| LEAP-Seq n nonconfirming: | 84 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCACCTTTCGCTCAGAGG |
| Suggested primer 2: | CACCAACTCCACTTCCACCT |