| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.106955 |
| Chromosome: | chromosome 14 |
| Location: | 195002 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g608990 | (1 of 6) PTHR13339:SF0 - COP9 SIGNALOSOME COMPLEX SUBUNIT 8 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCCGCCACCTCGGCAGAAGGCTGGCGC |
| Internal bar code: | TATCCAAACACCGGACAACCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 653 |
| LEAP-Seq percent confirming: | 98.9501 |
| LEAP-Seq n confirming: | 377 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGTTGGATGTATTGGGG |
| Suggested primer 2: | GGGTATGATAAGGCAGCGAA |