Insertion junction: LMJ.RY0402.106996_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.106996
Chromosome:chromosome 8
Location:2223976
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g369850 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCGTACAGCGCATCGGCACCGTACTCGT
Internal bar code:CGCTGAGCAACCGACAGAGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:70
LEAP-Seq percent confirming:77.3585
LEAP-Seq n confirming:246
LEAP-Seq n nonconfirming:72
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACCGTGTCGTACCACTCCTC
Suggested primer 2:TTCAACCTGGCCCTCTACAC