| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.107019 |
| Chromosome: | chromosome 2 |
| Location: | 2334434 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g090700 | MAPKKK8 | Mitogen-Activated Protein Kinase Kinase Kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCCTAGCAACTACACGCACCTGTGGCG |
| Internal bar code: | GGGGGAGATGGCGCTTCGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 98.6359 |
| LEAP-Seq n confirming: | 1880 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGCTGAGGTAGGGGTTG |
| Suggested primer 2: | ATGGTGAGTTGCTGCTGTTG |