Insertion junction: LMJ.RY0402.107063_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.107063
Chromosome:chromosome 12
Location:5490254
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g530900 (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAACAGTGTGACGCGATGGATGCGAAGTG
Internal bar code:AGAACAATCCGGCGGAGGCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:687
LEAP-Seq percent confirming:99.1608
LEAP-Seq n confirming:1418
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TCACGGAAGTGTGTGTTGGT
Suggested primer 2:CCACCTTTACACCCCTGCTA