| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.107085 |
| Chromosome: | chromosome 9 |
| Location: | 7052305 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411400 | MOT45 | PF13 protein required for dynein inner/outer arm assembly in the | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAATGACTTCCGCCGGCCCCGGTCCATGT |
| Internal bar code: | ACGCCCCGGTGCTAACCAAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 90.9237 |
| LEAP-Seq n confirming: | 95349 |
| LEAP-Seq n nonconfirming: | 9518 |
| LEAP-Seq n unique pos: | 183 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTACTGCCCTTCGTTCC |
| Suggested primer 2: | GTGAAGGAAAAGGCAATGGA |