| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.107089 |
| Chromosome: | chromosome 9 |
| Location: | 2354625 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392050 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGCGCCAGCACGGTGTCTGCGGCCTGGT |
| Internal bar code: | AGTGATATCACTGCTTGCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1135 |
| LEAP-Seq percent confirming: | 99.7353 |
| LEAP-Seq n confirming: | 8289 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTGAGCTCCTAAAGCGG |
| Suggested primer 2: | TACCCTCCACTCACCAGGTC |