Insertion junction: LMJ.RY0402.107104_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.107104
Chromosome:chromosome 14
Location:1677995
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g619200 (1 of 140) IPR002048 - EF-hand domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGAGCGCAACAGTTCGGCAAGGGGCAAGT
Internal bar code:GCCAAGTTCGGCAGTCTGCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:778
LEAP-Seq percent confirming:99.6751
LEAP-Seq n confirming:14420
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ATTTGTTCTACGCCTGGGTG
Suggested primer 2:TTCGCACTTTCTACCCTGCT