| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.107108 |
| Chromosome: | chromosome 2 |
| Location: | 801516 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078885 | (1 of 2) PTHR10997//PTHR10997:SF7 - IMPORTIN-7, 8, 11 // IMPORTIN-11 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTAAGAAGCGAGGGAGGGGGGGTCTGCCA |
| Internal bar code: | GTGGGTACCAGCAGGCTTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 245 |
| LEAP-Seq percent confirming: | 99.6308 |
| LEAP-Seq n confirming: | 5667 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGTGCTCGGCTAGTTAC |
| Suggested primer 2: | TGATCAGGCAGGGAGGTTAC |