| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.107140 |
| Chromosome: | chromosome 16 |
| Location: | 5520721 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680500 | EFH10 | (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair; EF-hand Calcium binding protein, may be centrin-related | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAACGAATGAGTGAAAGTTTCGCCACA |
| Internal bar code: | GTGGTGGCTCCGGCCCCATGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 599 |
| LEAP-Seq percent confirming: | 99.0364 |
| LEAP-Seq n confirming: | 925 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGCGTTGGTGTAGTGGT |
| Suggested primer 2: | ACCATATTATCCCGGAAGGC |