| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.107146 |
| Chromosome: | chromosome 16 |
| Location: | 6263359 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674850 | (1 of 1) PTHR14625//PTHR14625:SF3 - MICROCEPHALIN // MICROCEPHALIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATGCCGTGCAGCCAGCGCGACTGACGC |
| Internal bar code: | AGACCTGGACGGTCTGCTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 174 |
| LEAP-Seq percent confirming: | 99.7273 |
| LEAP-Seq n confirming: | 1097 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTACGTCACGTCGCACTGT |
| Suggested primer 2: | GTGGGTGGGGTGATTTGTAG |