| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.107152 |
| Chromosome: | chromosome 13 |
| Location: | 41801 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g562100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCCAATGAGCGGCATGGCATGGACAATC |
| Internal bar code: | CCCTAAACCCTCGAGGAGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 80 |
| LEAP-Seq percent confirming: | 92.9032 |
| LEAP-Seq n confirming: | 144 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGTGTGTGTTTGCTTGGG |
| Suggested primer 2: | AGGGCATAGACACAACGGAC |