Insertion junction: LMJ.RY0402.107156_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.107156
Chromosome:chromosome 3
Location:451735
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g145027 (1 of 14) PF13371 - Tetratricopeptide repeat (TPR_9) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAAAGCGCACACCCCACACGTCTGGGGTG
Internal bar code:TGGATATTTCTCGGCAGCTATC

Confirmation - LEAP-Seq

LEAP-Seq distance:746
LEAP-Seq percent confirming:99.0379
LEAP-Seq n confirming:1647
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCATCACCCTTTCTCCATGT
Suggested primer 2:GACACTGGCATTGTTGGATG