Insertion junction: LMJ.RY0402.107166_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.107166
Chromosome:chromosome 14
Location:3820078
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g632700 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACCTGGCACGAGCATGCAGCCAGTACAC
Internal bar code:GTGGAGGGGGGCCGATTCATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:738
LEAP-Seq percent confirming:98.8959
LEAP-Seq n confirming:10301
LEAP-Seq n nonconfirming:115
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGGAAATGGGAAAGAACC
Suggested primer 2:CCATCAAGACACTGGTGGTG