| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.107167 |
| Chromosome: | chromosome 12 |
| Location: | 4666989 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523150 | PFD5 | Prefoldin alpha subunit; (1 of 1) K04797 - prefoldin alpha subunit (pfdA, PFDN5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCAGTTGGGCGGGAGTCCAGCGTGCGG |
| Internal bar code: | GATGAGGCGCTGACCACACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1129 |
| LEAP-Seq percent confirming: | 98.8605 |
| LEAP-Seq n confirming: | 6854 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTGCCTACAGGTCGCTT |
| Suggested primer 2: | GTTCAGGGTCTGCAAGAAGC |