Insertion junction: LMJ.RY0402.107179_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.107179
Chromosome:chromosome 3
Location:1234979
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g149800 TOG1 (1 of 1) K16803 - cytoskeleton-associated protein 5 (CKAP5, XMAP215); Microtubule associated protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGCGATCGACCTAATGGTACAGCACATC
Internal bar code:GATCAGCTACAACATCGAGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:239
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:138
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AACTGCTGCACATCATCTCG
Suggested primer 2:GATCATCATCTCCAGCAGCA