| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.107184 |
| Chromosome: | chromosome 17 |
| Location: | 225953 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACGCGCGGCCCCACCACCAGCCGGCTTA |
| Internal bar code: | GTTCCATTTTTTCTTCCGACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 333 |
| LEAP-Seq percent confirming: | 99.4632 |
| LEAP-Seq n confirming: | 1853 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATATGTGCAGTTGTTGCC |
| Suggested primer 2: | GGGTTGTGTGCATTGATCTG |