| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.107185 |
| Chromosome: | chromosome 12 |
| Location: | 4492748 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521750 | NUP133 | Nucleoporin 133; (1 of 2) PF08801 - Nup133 N terminal like (Nucleoporin_N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCCTCCGCGCCACTCACTGCGTGTGGC |
| Internal bar code: | GGGGTTTTAAGGGATCGGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 55 |
| LEAP-Seq percent confirming: | 82.6087 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGCAAAGTGCGGTAGTA |
| Suggested primer 2: | CCAGTGTGTCCTTGCTGCTA |