| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.107194 |
| Chromosome: | chromosome 9 |
| Location: | 6561112 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408051 | (1 of 2) K15032 - mTERF domain-containing protein, mitochondrial (MTERFD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGTCATCCCCACCCCCACCCCCACACAC |
| Internal bar code: | GGGGTCGCTGTGAACAAGCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 96.0807 |
| LEAP-Seq n confirming: | 1667 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGGGAACCTGGGAATAC |
| Suggested primer 2: | CCATGTTGCTGACTACCCCT |