| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.107199 |
| Chromosome: | chromosome 12 |
| Location: | 3946828 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516333 | (1 of 10) PF07460 - NUMOD3 motif (2 copies) (NUMOD3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCCTTCCCGGCCCCTGCGCCGCCCTTG |
| Internal bar code: | CGTCGGTAATTGTAATCTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 97.6116 |
| LEAP-Seq n confirming: | 1553 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTTGCAGGAGCTGGC |
| Suggested primer 2: | TCCTCCTCATCGTCCTCATC |