| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.107213 |
| Chromosome: | chromosome 6 |
| Location: | 1997342 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264100 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCGTCGTTCGATAGTACGCTAAACTAAA |
| Internal bar code: | TCCAAGCACTTTTAGCAATTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1180 |
| LEAP-Seq percent confirming: | 96.8332 |
| LEAP-Seq n confirming: | 1376 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACCACAGAGAAGATGCAA |
| Suggested primer 2: | AGTCGGAATGAAATGATGGC |