| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.107220 |
| Chromosome: | chromosome 2 |
| Location: | 2455452 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091850 | (1 of 1) 4.2.1.18 - Methylglutaconyl-CoA hydratase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAAGCCCATGCCAGGTCGCAACCATCCC |
| Internal bar code: | GCCGCGGGTAATCTTCGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 239 |
| LEAP-Seq percent confirming: | 99.6644 |
| LEAP-Seq n confirming: | 594 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACATCCGGAAACGAGTC |
| Suggested primer 2: | AGCCTGCTCTGGTGAGTCAT |