| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.107223 |
| Chromosome: | scaffold 26 |
| Location: | 53256 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre26.g756947 | (1 of 1) PF15253 - SCL-interrupting locus protein N-terminus (STIL_N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGTGCAACCGCCTGACCGAGTGCTTCG |
| Internal bar code: | GAGTGGAAGTGGGTGGTCGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 221 |
| LEAP-Seq percent confirming: | 99.1263 |
| LEAP-Seq n confirming: | 8055 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCATAATGAGCCGCGTAAC |
| Suggested primer 2: | CAACACCACCTCCAACAACA |