| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.107234 |
| Chromosome: | chromosome 3 |
| Location: | 2119210 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157000 | ELG1 | Exostosin-like glycosyltransferase 1; (1 of 1) PF12661 - Human growth factor-like EGF (hEGF) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGACACAATCATGCAGTGGCTCTACTCG |
| Internal bar code: | TAGTAACCTTGTATAGGCGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 713 |
| LEAP-Seq percent confirming: | 98.9026 |
| LEAP-Seq n confirming: | 3695 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACGTGCACTCCTTAGTCA |
| Suggested primer 2: | CAGGCCACGTAAATCAAGGT |