| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.107256 |
| Chromosome: | chromosome 17 |
| Location: | 7028375 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746647 | PUF2 | (1 of 1) PTHR12537:SF12 - MATERNAL PROTEIN PUMILIO; Puf protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGATTGCGAGGGCAGGGAGGGGGGGAAGC |
| Internal bar code: | ACTGCCCTTCGAGTGGCATCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 95.9103 |
| LEAP-Seq n confirming: | 727 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCCAGAAGTGCATCGAG |
| Suggested primer 2: | GCTTGCTAGGCCATTACTGC |