| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.107257 |
| Chromosome: | chromosome 3 |
| Location: | 6695709 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197500 | MOT6 | (1 of 1) PTHR10891//PTHR10891:SF554 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN // SUBFAMILY NOT NAMED; Predicted protein | 5'UTR_intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAATCTAATCGAACAAGCACGTTACCGC |
| Internal bar code: | TGTTTTTCTGCACCCAAGAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 96.2389 |
| LEAP-Seq n confirming: | 870 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCAAGCACAAGCACAGCC |
| Suggested primer 2: | GCAAGGTGGAAAAAGCACAT |