Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.107261 |
Chromosome: | chromosome_16 |
Location: | 1201779 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g650750 | FAP268 | Flagella Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGCCCCAACCGCAGGGCAGGGTTCGTGTCG |
Internal bar code: | GTTCCCCTTCGGCATGCAAGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 489 |
LEAP-Seq percent confirming: | 98.6301 |
LEAP-Seq n confirming: | 72 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGTGAGTCCTAGCCAAGCC |
Suggested primer 2: | GGAACCACAGCATTGACCTT |