Insertion junction: LMJ.RY0402.107267_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.107267
Chromosome:chromosome 4
Location:2903952
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g224550 GT90F37,GT90-37 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 37 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACATTCCTGTGGATCCAGACCAGTGCGG
Internal bar code:CTCGGCTTTGGAGCCGCCAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:951
LEAP-Seq percent confirming:98.8593
LEAP-Seq n confirming:260
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTGCTGCTTCATGTCGTC
Suggested primer 2:ACACCCCCATCCCTCTTATC